Aspirin use, 8q24 single nucleotide polymorphism rs6983267, and colorectal cancer according to ctnnb1 alterations

62Citations
Citations of this article
82Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

BackgroundRegular aspirin use reduces the risk for colorectal cancer (CRC), possibly through inhibition of WNT/cadherin-associated protein β1 (CTNNB1 or β-catenin) signaling. The single nucleotide polymorphism (SNP) rs6983267 on chromosome 8q24 is a CRC susceptibility locus that affects binding activity of transcription factor 7 like-2 (TCF7L2) to CTNNB1, thereby altering expression of target oncogenes, including MYC.MethodsWe evaluated regular aspirin use and CRC risk according to genotypes of SNP rs6983267 and CTNNB1 expression status in two prospective case-control studies (840 CRC case patients and 1686 age-and race-matched control subjects) nested within the Nurses' Health Study and the Health Professionals Follow-up Study. We estimated odds ratios (ORs) and 95% confidence intervals (CIs) using unconditional logistic regression models. All statistical tests were two-sided.ResultsA lower risk of CRC was associated with regular aspirin use and the T allele of rs6983267. The effect of aspirin was confined to individuals with protective T allele of rs6983267 (additive matching factors-adjusted OR for T allele = 0.83; 95% CI = 0.74 to 0.94; P trend =. 002; Pinteraction =. 01). Additionally, the T allele of rs6983267 was associated with a reduced expression of MYC oncogene (Ptrend =. 03). Moreover, among individuals with protective T allele, the effect of regular aspirin use was limited to those with positive nuclear CTNNB1 expression. In a functional analysis, in vitro treatment of LS174T cells (a cell line heterozygous for rs6983267) with aspirin was statistically significantly associated with higher G/T allelic ratio of TCF7L2 immunoprecipitated DNA (P =. 03).ConclusionsOur results support an influence of aspirin on WNT/CTNNB1 signaling and suggest that aspirin chemoprevention may be tailored according to rs6983267 genotype. © 2013 The Author .

Cite

CITATION STYLE

APA

Nan, H., Morikawa, T., Suuriniemi, M., Imamura, Y., Werner, L., Kuchiba, A., … Chan, A. T. (2013). Aspirin use, 8q24 single nucleotide polymorphism rs6983267, and colorectal cancer according to ctnnb1 alterations. Journal of the National Cancer Institute, 105(24), 1852–1861. https://doi.org/10.1093/jnci/djt331

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free