Association between SUMO4 polymorphisms and coronary artery disease with and without type 2 diabetes mellitus

Abstract

Objective: To assess the role of small ubiquitin-like modifier 4 (SUMO4) gene polymorphisms (rs237025,rs237024 and rs600739) in the susceptibility to coronary artery disease (CAD) with and without type 2 diabetes mellitus (T2DM) in Chinese Han ethnic population in Beijing. Methods: In this case-control study, 558 subjects with angiography-proven CAD were divided into two groups according to the WHO 1999 criteria, 369 with normal glucose tolerance (CAD group) and 189 with T2DM (T2DM + CAD group). Meanwhile 500 healthy subjects free of T2DM and CAD were selected as normal controls (control group). Allelic and genotypic distributions of the three single nucleotide polymorphisms (SNPs) were determined with polymerase chain reaction-high resolution melting curve (PCR-HRM) and gene sequencing. Clinical and biochemical data were compared among carriers of different genotypes through a stratified analysis. Results: No significant difference was found in the distribution of genotypes and alleles of each SNP between different groups (P>0.05). Nevertheless, stratified analysis indicated a significant difference in plasma triglycerides (rs237025) and body mass index (rs600739) among individuals of different genotypes from the T2DM + CAD group (P = 0.020 and P = 0.049, respectively). Multiple comparison also indicated that GG genotype of rs237025 had a higher level of plasma triglycerides than AA genotype (P< 0.01). Conclusion: No association between SUMO4 gene polymorphisms and CAD with and without T2DM was detected. Such polymorphisms may not be a risk factor for Chinese Han ethnic patients in Beijing.