Axenically culturing the bryophytes: a case study of the moss Dicranum scoparium Hedw. (Dicranaceae, Bryophyta)

Abstract

The aim of this study was to establish axenic culture of the moss Dicranum scoparium a counterpart of rare and widly endangered D. viride. T e media contents, as well as light and temperature were varied to fi nd the optimal conditions for spore germination, protonema growing, bud formation and gametophyte development. T e best contitions for micropropagation or axenically bryo-farming is to grow D. scoparium on the MS medium enriched with sucrose (1.5%), at 18-20°C independent of light length condition.