Bacterial colonization of functionalized polyurethanes

Abstract

A protocol was developed for studying the growth of bacteria upon polyurethanes subsequent to the establishment of an adherent bacterial population. An inocula of approximately 105cfu S. aureus were spread on functionalized polyurethanes which included Pellethane, sulfonated Pellethane, phosphonated Pellethane, quaternized amine polyurethanes, and a zwitterionic phosphonated polyurethane. After 24h incubation, Pellethane, sulfonated Pellethane, and phosphonated Pellethane showed bacterial growth by at least a factor of 10. In contrast, the zwitterionic phosphonated polyurethane showed a factor of 10 decrease in bacteria after 24h and the quaternized amine polyurethanes reduced the bacteria to only a few hundred after only 1h. When treated with bovine serum albumin, Pellethane, sulfonated Pellethane, and phosphonated Pellethane again showed bacterial growth by as much as a factor of 10 over 24h. The quaternized amine polyurethanes and the zwitterionic phosphonated polyurethane still exhibited bactericidal abilities even when coated with bovine serum albumin, with the zwitterionic material reducing bacteria by more than a factor of 10 over 24h and the quaternized amine polyurethane reducing the bacteria to only a few hundred after only 1h. A zone of inhibition study suggested that the bactericidal activity of the zwitterionic phosphonated polyurethane was due to the leaching of cadmium ions. A quaternized amine polyurethane which contained chloride instead of iodide as the counterion to the amine moiety was less bactericidal than the iodide-containing polymer when treated with albumin. Thus, bacteria were able to colonize Pellethane, phosphonated sulfonated Pellethane, and phosphonated Pellethane, but the iodide-containing quaternized amine polyurethane and the zwitterionic polyurethane prevented colonization. Copyright (C) 1999 Elsevier Science B.V.