Sign up & Download
Sign in

A comparison between dual polarization interferometry (DPI) and surface plasmon resonance (SPR) for protein adsorption studies

by Andreas W. Sonesson, Thomas H. Callisen, Hjalmar Brismar, Ulla M. Elofsson
Colloids and Surfaces B: Biointerfaces ()

Abstract

This work was performed with the aim of comparing protein adsorption results obtained from the recently developed dual polarization interferometry (DPI) with the well-established surface plasmon resonance (SPR) technique. Both techniques use an evanescent field as the sensing element but completely different methods to calculate the adsorbed mass. As a test system we used adsorption of the lipase from Thermomyces lanuginosus (TLL) on C18 surfaces. The adsorbed amount calculated with both techniques is in good agreement, with both adsorption isotherms saturating at 1.30-1.35 mg/m2 at TLL concentrations of 1000 nM and above. Therefore, this supports the use of both SPR and DPI as tools for studying protein adsorption, which is very important when comparing adsorption data obtained from the use different techniques. Due to the spot sensing in SPR, this technique is recommended for initial kinetic studies, whereas DPI is more accurate when the refractive index and thickness of the adsorbed layer is of more interest. © 2006 Elsevier B.V. All rights reserved.

Cite this document (BETA)

Page 1
hidden
Page 2
hidden

Readership Statistics

22 Readers on Mendeley
by Discipline
 
 
 
by Academic Status
 
27% Post Doc
 
27% Ph.D. Student
 
14% Student (Master)
by Country
 
14% United Kingdom
 
9% Hungary

Sign up today - FREE

Mendeley saves you time finding and organizing research. Learn more

  • All your research in one place
  • Add and import papers easily
  • Access it anywhere, anytime

Start using Mendeley in seconds!

Already have an account? Sign in