The alkaline phosphatase immunoassay (APIA) is an antibody detection technique which permits the diagnosis of schistosomiasis rising a butanolic extract preparation from adult worms. APIA has demonstrated high sensitivity and specificity in previous reports with well characterized human sera. Its potential as a diagnostic tool for epidemiological surveillance was assessed in comparison with three other diagnostic tests: stool examination, ELISA with soluble egg antigen (SEA) and the circumoval precipitin test (COPT). APIA was 100% specific in an area without Schistosoma mansoni transmission and had 89% sensitivity in an endemic area where 69% of the infected subjects excreted less than 100 eggs/g of faeces. It was found to be less sensitive in children under 5 years of age who were positive by the COPT test. APIA can be applied as an initial screening test, based on its high sensitivity, specificity, absence of cross-reactivity with intestinal parasites and the fact that it is a technique suitable for use in epidemiological surveillance.
CITATION STYLE
Alarcón De Noya, B., Cesari, I. M., Losada, S., Colmenares, C., Balzán, C., Hoebeke, J., & Noya, O. (1997). Evaluation of alkaline phosphatase immunoassay comparison with other diagnostic methods in areas of low transmission of schistosomiasis. Acta Tropica, 66(2), 69–78. https://doi.org/10.1016/S0001-706X(97)00032-6
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