This study aims to explore the genotypes and drug susceptibility of Trichosporon asahii (T. asahii) to common antifungal drugs in vitro. Ten strains of Trichosporon were identified with API 20C AUX and Vitek 2 Compact according to morphology. Genotypes were identified by rDNA ITS, 26S rRNA PCR amplification, and sequence analysis. The minimum inhibitory concentration (MIC) of antifungal drugs was detected by E-test assay. The ten strains of Trichosporon were T. asahii. The strains were divided into three genotypes, namely, genotype IV (n = 7), genotype I (n = 2), and genotype III (n = 1), through sequence analysis. Susceptibility test showed that voriconazole and itraconazole, with MICs of 0.08 and 0.7 μg/ml, respectively, exhibited the best inhibitory activity against T. asahii. rDNA ITS sequence analysis can be utilized to identify Trichosporon strains. 26S rRNA IGS 1 genotyping can be utilized in epidemiological investigations. Voriconazole and itraconazole can be employed to treat Trichosporon infections because these drugs exhibit effective inhibitory activity against Trichosporon.
CITATION STYLE
Jiang, W., Li, S., Zhai, L., & Yu, Y. (2013). Facility Location and Supply Chain Management – a Review. Life Science Journal, 10(3), 1226–1230. Retrieved from https://www.sciencedirect.com/science/article/pii/S0377221708004104
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