Methods for analysis of single nucleotide polymorphisms (SNP) are well developed. However, most ready-made SNP genotyping kits (e.g., those that use TaqMan® PCR) are based on the assumption that the SNP is biallelic. Thus, most kits are unsuitable for triallelic SNPs. We have experienced difficulty genotyping an SNP using TaqMan® PCR. In the present study, we developed a method of genotyping a triallelic SNP (rs3091244: alleles C, A and T) using TaqMan® PCR. We used 2 different genotyping kits: one for C/A allele genotyping, and one for C/T allele genotyping. The results of these 2 kits were combined to complete the genotyping. The subjects were 320 essentially healthy elderly Japanese. The frequencies of the C, A and T alleles were 0.78, 0.155 and 0.065, respectively. This double-tube method using TaqMan® SNP Genotyping Assays was very accurate and convenient, and it should be useful for genotyping in case-control association studies or linkage studies. © 2006 Elsevier Ltd. All rights reserved.
CITATION STYLE
Morita, A., Nakayama, T., Doba, N., Hinohara, S., Mizutani, T., & Soma, M. (2007). Genotyping of triallelic SNPs using TaqMan® PCR. Molecular and Cellular Probes, 21(3), 171–176. https://doi.org/10.1016/j.mcp.2006.10.005
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