The Escherichia coli H-NS protein is a nucleoid-associated protein involved in gene regulation and DNA compaction. To get more insight into the mechanism of DNA compaction we applied atomic force microscopy (AFM) to study the structure of H-NS-DNA complexes. On circular DNA molecules two different levels of H-NS induced condensation were observed. H-NS induced lateral condensation of large regions of the plasmid. In addition, large globular structures were identified that incorporated a considerable amount of DNA. The formation of these globular structures appeared not to be dependent on any specific sequence. On the basis of the AFM images, a model for global condensation of the chromosomal DNA by H-NS is proposed.
CITATION STYLE
Dame, R. T., Wyman, C., & Goosen, N. (2000). H-NS mediated compaction of DNA visualised by atomic force microscopy. Nucleic Acids Research, 28(18), 3504–3510. https://doi.org/10.1093/nar/28.18.3504
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