A synthetic peptide with the amino acid sequence 135-154 of the capsid protein VP1 of the foot-and-mouth-disease virus was modified with ω-hydroxyundecanethiol and applied together with non-derivatised ω-hydroxyundecanethiol for consecutive adsorption onto gold electrodes according to self-assembling procedures. The binding of a specific antibody to prepared recognition layers could be monitored by measurement of impedance or capacitance. In order to avoid non-specific effects, all measurements were performed in the presence of BSA. The complex between the antigenic peptide and the antibody was split by applying 6 M urea solution. The gold electrodes were mounted into an optimised flow-through system in order to perform capacitance-time measurements. The immobilised peptide can be recognised repeatedly by specific antibodies.
CITATION STYLE
Rickert, J., Göpel, W., Beck, W., Jung, G., & Heiduschka, P. (1996). A “mixed” self-assembled monolayer for an impedimetric immunosensor. In Biosensors and Bioelectronics (Vol. 11, pp. 757–768). Elsevier Science Ltd. https://doi.org/10.1016/0956-5663(96)85927-6
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