Nascent-Seq reveals novel features of mouse circadian transcriptional regulation

242Citations
Citations of this article
225Readers
Mendeley users who have this article in their library.

Abstract

A substantial fraction of the metazoan transcriptome undergoes circadian oscillations in many cells and tissues. Based on the transcription feedback loops important for circadian timekeeping, it is commonly assumed that this mRNA cycling reflects widespread transcriptional regulation. To address this issue, we directly measured the circadian dynamics of mouse liver transcription using Nascent-Seq (genome-wide sequencing of nascent RNA). Although many genes are rhythmically transcribed, many rhythmic mRNAs manifest poor transcriptional rhythms, indicating a prominent contribution of post-transcriptional regulation to circadian mRNA expression. This analysis of rhythmic transcription also showed that the rhythmic DNA binding profile of the transcription factors CLOCK and BMAL1 does not determine the transcriptional phase of most target genes. This likely reflects gene-specific collaborations of CLK:BMAL1 with other transcription factors. These insights from Nascent-Seq indicate that it should have broad applicability to many other gene expression regulatory issues. © Menet et al.

Cite

CITATION STYLE

APA

Menet, J. S., Rodriguez, J., Abruzzi, K. C., & Rosbash, M. (2012). Nascent-Seq reveals novel features of mouse circadian transcriptional regulation. ELife, 2012(1). https://doi.org/10.7554/eLife.00011

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free