Difficulties were encountered in labeling 5'-aminoalkyl DNA oligomers with glycolketo electrophore N-hydroxy-succinimide esters in aqueous sodium bicarbonate (a common base for this purpose), followed by C18-silica reversed-phase high-performance liquid chromatography (HPLC) to achieve purification. The electrophore-labeled oligomers were not separated readily either from the hydrolyzed electrophore or from the starting oligomer. This problem was overcome by conducting the reaction with triethylamine as a base, organic washing the reaction mixtures after evaporation, and separating on a C18-poly(styrene-divinylbenzene) HPLC packing.
CITATION STYLE
Wang, Z., Bian, N., Qian, X., & Giese, R. W. (1998). N-hydroxysuccinimide ester labeling 5’-aminoalkyl DNA oligomers: Reaction conditions and purification. Journal of Chromatography A, 806(1), 93–95. https://doi.org/10.1016/S0021-9673(98)00093-4
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