The potential value of PCRs in the species-specific diagnosis of Taenia saginata and Taenia solium have been investigated, using samples of T. saginata and T. solium from different geographical areas. The PCRs examining inter-species differences were based on the sequence of the HDP2 DNA fragment, specific for T. saginata/T. solium, and the sequence of the rDNA internal transcribed spacer 1 and spacer 2 (ITS-1 and ITS-2). This PCR analysis of DNA isolates confirmed morphologic diagnosis and allowed the speciation of samples too small or fragmented for morphologic identification, with clear and consistent inter-species differences between T. saginata (twenty-two) and T. solium (three) geographical isolates. Possible intra-species genomic variability, within these species, was similarly studied through analysis of PCR amplification products (PCR-RFLP) and only encountered one exceptional T. saginata isolate from Kenya, which yielded a unique PCR-RFLP pattern, different from T. saginata DNA of Mexican (one sample) and Spanish (seven samples) origin. © 2002 Elsevier Science Inc. All rights reserved.
CITATION STYLE
Miguel González, L., Montero, E., Puente, S., López-Velez, R., Hernández, M., Sciutto, E., … Gárate, T. (2002). PCR tools for the differential diagnosis of Taenia saginata and Taenia solium taeniasis/cysticercosis from different geographical locations. Diagnostic Microbiology and Infectious Disease, 42(4), 243–249. https://doi.org/10.1016/S0732-8893(01)00356-X
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