Successful cancer therapy requires the elimination or incapacitation of all tumor cells capable of regenerating a tumor. Therapeutic advances therefore necessitate the characterization of the cells that are able to propagate a tumor in vivo. We show an important link between tumor genotype and isolation of tumor-propagating cells (TPCs). Three mouse models of the most common form of human lung cancer each had TPCs with a unique cell-surface phenotype. The cell-surface marker Sca1 did not enrich for TPCs in tumors initiated with oncogenic Kras, and only Sca1-negative cells propagated EGFR mutant tumors. In contrast, Sca1-positive cells were enriched for tumor-propagating activity in Kras tumors with p53 deficiency. Primary tumors that differ in genotype at just one locus can therefore have tumor-propagating cell populations with distinct markers. Our studies show that the genotype of tumor samples must be considered in studies to identify, characterize, and target tumor-propagating cells. © 2010 Elsevier Inc.
CITATION STYLE
Curtis, S. J., Sinkevicius, K. W., Li, D., Lau, A. N., Roach, R. R., Zamponi, R., … Kim, C. F. (2010). Primary tumor genotype is an important determinant in identification of lung cancer propagating cells. Cell Stem Cell, 7(1), 127–133. https://doi.org/10.1016/j.stem.2010.05.021
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