We describe a method based on Rosetta structure refinement for generating high-resolution, all-atom protein models from electron cryomicroscopy density maps. A local measure of the fit of a model to the density is used to directly guide structure refinement and to identify regions incompatible with the density that are then targeted for extensive rebuilding. Over a range of test cases using both simulated and experimentally generated data, the method consistently increases the accuracy of starting models generated either by comparative modeling or by hand-tracing the density. The method can achieve near-atomic resolution starting from density maps at 4-6 Å resolution. © 2009 Elsevier Ltd. All rights reserved.
CITATION STYLE
DiMaio, F., Tyka, M. D., Baker, M. L., Chiu, W., & Baker, D. (2009). Refinement of Protein Structures into Low-Resolution Density Maps Using Rosetta. Journal of Molecular Biology, 392(1), 181–190. https://doi.org/10.1016/j.jmb.2009.07.008
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