RNA-Seq: A revolutionary tool for transcriptomics

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Abstract

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes. © 2009 Macmillan Publishers Limited. All rights reserved.

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Wang, Z., Gerstein, M., & Snyder, M. (2009, January). RNA-Seq: A revolutionary tool for transcriptomics. Nature Reviews Genetics. https://doi.org/10.1038/nrg2484

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