A method for the determination of metoprolol and its main metabolises in urine is presented. The method comprises derivatization of the aminopropanol side-chain with phosgene at alkaline pH and isolation in an organic phase at acidic pH. After trimethylsilylation, separation and quantification are performed by capillary column gas chromatography with flame ionization detection. The reaction is performed at pH 12 with 60 μl of 2 M phosgene in toluene added in three portions. Diethyl ether-dichloromethane is used as extraction medium and bis(trimethylsilyl)acetamide as silylating agent. With spiked samples linear standard curves were obtained for metoprolol and three of its main metabolises with a detection limit varying between 4 and 20 μol/l of urine. The method was applied to urine samples from a normal individual who had taken 292 μmol of metoprolol as tartrate. © 1984.
CITATION STYLE
Gyllenhaal, O., & Hoffmann, K. J. (1984). Simultaneous determination of metoprolol and metabolites in urine by capillary column gas chromatography as oxazolidineone and trimethylsilyl derivatives. Journal of Chromatography B: Biomedical Sciences and Applications, 309(C), 317–328. https://doi.org/10.1016/0378-4347(84)80039-0
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