Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with desulfovibrio vulgaris hildenborough and methanobacterium congolense: Global transcriptomic and proteomic analyses

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Abstract

Dehalococcoides ethenogenes strain 195 (DE195) was grown in a sustainable syntrophic association with Desulfovibrio vulgaris Hildenborough (DVH) as a co-culture, as well as with DVH and the hydrogenotrophic methanogen Methanobacterium congolense (MC) as a tri-culture using lactate as the sole energy and carbon source. In the co- and tri-cultures, maximum dechlorination rates of DE195 were enhanced by approximately three times (11.0±0.01 μmol per day for the co-culture and 10.1±0.3 μmol per day for the tri-culture) compared with DE195 grown alone (3.8±0.1 μmol per day). Cell yield of DE195 was enhanced in the co-culture (9.0±0.5 × 10 7 cells per mol Cl - released, compared with 6.8±0.9 × 10 7 cells per μmol Cl - released for the pure culture), whereas no further enhancement was observed in the tri-culture (7.3±1.8 × 10 7 cells per μmol Cl - released). The transcriptome of DE195 grown in the co-culture was analyzed using a whole-genome microarray targeting DE195, which detected 102 significantly up- or down-regulated genes compared with DE195 grown in isolation, whereas no significant transcriptomic difference was observed between co- and tri-cultures. Proteomic analysis showed that 120 proteins were differentially expressed in the co-culture compared with DE195 grown in isolation. Physiological, transcriptomic and proteomic results indicate that the robust growth of DE195 in co- and tri-cultures is because of the advantages associated with the capabilities of DVH to ferment lactate to provide H 2 and acetate for growth, along with potential benefits from proton translocation, cobalamin-salvaging and amino acid biosynthesis, whereas MC in the tri-culture provided no significant additional benefits beyond those of DVH. © 2012 International Society for Microbial Ecology All rights reserved.

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Men, Y., Feil, H., Verberkmoes, N. C., Shah, M. B., Johnson, D. R., Lee, P. K. H., … Alvarez-Cohen, L. (2012). Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with desulfovibrio vulgaris hildenborough and methanobacterium congolense: Global transcriptomic and proteomic analyses. ISME Journal, 6(2), 410–421. https://doi.org/10.1038/ismej.2011.111

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