Cathepsins B and L contribute to Ebola virus (EBOV) entry into Vero cells and mouse embryonic fibroblasts. However, the role of cathepsins in EBOV-infection of human dendritic cells (DCs), important targets of infection in vivo, remains undefined. Here, EBOV-like particles containing a β-lactamase-VP40 fusion reporter and Ebola virus were used to demonstrate the cathepsin dependence of EBOV entry into human monocyte-derived DCs. However, while DC infection is blocked by cathepsin B inhibitor, it is insensitive to cathepsin L inhibitor. Furthermore, DCs pre-treated for 48 h with TNFα were generally less susceptible to entry and infection by EBOV. This decrease in infection was associated with a decrease in cathepsin B activity. Thus, cathepsin L plays a minimal, if any, role in EBOV infection in human DCs. The inflammatory cytokine TNFα modulates cathepsin B activity and affects EBOV entry into and infection of human DCs. © 2009 Blackwell Publishing Ltd.
CITATION STYLE
Martinez, O., Johnson, J., Manicassamy, B., Rong, L., Olinger, G. G., Hensley, L. E., & Basler, C. F. (2010). Zaire Ebola virus entry into human dendritic cells is insensitive to cathepsin L inhibition. Cellular Microbiology, 12(2), 148–157. https://doi.org/10.1111/j.1462-5822.2009.01385.x
Mendeley helps you to discover research relevant for your work.