Background: The filamentous fungus Aspergillus niger is widely exploited as an important expression host for industrial production. The glucoamylase high-producing strain A. niger CICC2462 has been used as a host strain for the establishment of a secretion expression system. It expresses recombinant xylanase, mannase and asparaginase at a high level, but some high secretory background proteins in these recombinant strains still remain, such as alpha-amylase and alpha-glucosidase; lead to a low-purity of fermentation products. The aim was to construct an A. niger host strain with a low background of protein secretion. Results: The transcription factor amyR was deleted in A. niger CICC2462, and the results from enzyme activity assays and SDS-PAGE analysis showed that the glucoamylase and amylase activities of the δamyR strains were significantly lower than those of the wild-type strain. High-throughput RNA-sequencing and shotgun LC-MS/MS proteomic technology analysis demonstrated that the expression of amylolytic enzymes was decreased at both the transcriptional and translational levels in the δ amyR strain. Interestingly, the δ amyR strain growth rate better than the wild-type strain. Conclusions: Our findings clearly indicated that the δ amyR strain of A. niger CICC2462 can be used as a host strain with a low background of protein secretion.
CITATION STYLE
Zhang, H., Wang, S., Zhang, X. xiang, Ji, W., Song, F., Zhao, Y., & Li, J. (2016). The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background. Microbial Cell Factories, 15(1). https://doi.org/10.1186/s12934-016-0463-1
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