The in vitro generation of human cytotoxic T lymphocytes (CTL) is a reliably approach useful not only to assess intrinsic CD8+ T cell responses in individuals but also to screen immunogenic antigens that could be considered as candidates for adoptive immunotherapeutic approaches. In vitro methods to expand CTL require culturing naïve T cells with antigen-presenting cells (APC) as stimulator cells that express specific antigens. Here, we describe the protocol for generating CTL against target antigens presented by monocyte-derived dendritic cells (DCs).
CITATION STYLE
Gigante, M., & Ranieri, E. (2014). In Vitro\Ex Vivo generation of cytotoxic T lymphocytes. Methods in Molecular Biology, 1186, 13–20. https://doi.org/10.1007/978-1-4939-1158-5_2
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