In Vitro Selection Collection

In this group: 16 papers · 6 members

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Biological Sciences

Recent and classic advances in selection procedures for functional nucleic acids and protein.

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Christopher VanLang added documents to this group

Directed evolution of ATP binding proteins from a zinc finger domain by using mRNA display.

The use of mRNA display to select high-affinity protein-binding peptides.

Functional proteins from a random-sequence library.

20th April

Christopher VanLang added a document to this group

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution.

31st December, 2011

Federico Carrion is now following this group

22nd December, 2011

Christopher VanLang added documents to this group

Directed evolution of polymerase function by compartmentalized self-replication.

A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system.

Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenases.

Selection of ribozymes that catalyse multiple-turnover Diels-Alder cycloadditions by using in vitro compartmentalization.

In vitro selection of RNA molecules that bind specific ligands.

And 1 more

14th December, 2011

luis reyes

I´ve seen that most of papers use SPR or CE for monitoring apt-protein binding. Im looking for a cheapest alternative, i thought dot-blot could partially resolve this but im not sure about the protocol i need since i have my library labeled with FAM-5´. Any coment

17th November, 2011

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Simon Trevino anytime- there are many examples of simple protein-aptamer in vitro selections that are based on affinity chromatography. in most cases, the protein is expressed in some cell line, isolated, and immobilized on any matrix. bound aptamers can be recovered competitively with free protein, or by denaturants after non-binding nucleic acid has been washe...

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20th November

luis reyes thanks Simon...other question, im looking for a posdoctoral position in a group that deals wiht the SELEX method. So i would like to know if there any position available at your lab.

21st November

luis reyes Hi simon, thank you for your comments. I ´ve seen your profile as well as of your advisor. Actually i´ve sent an e-mail asking on postdoctoral opportunities, i guess he´s too busy at the moment,so that, i decided asking you...as a colleague.
i hope we can keep contact!
thanks.

21st November

luis reyes

im looking for protocols using CNBr-Sepaharose beads for purifying protein-aptamer complex. Thanks for your help.

12th September, 2011

Simon Trevino added a document to this group

In vitro selection of cathepsin E-activity-enhancing peptide aptamers at neutral pH

12th September, 2011

luis reyes thanks Simon!

17th November

luis reyes has joined this group

12th September, 2011

Amos Yan has joined this group

12th August, 2011

Christopher VanLang

It seems like I've accidentally joined an internal lab group... still mind if I contribute?

28th July, 2011

Simon Trevino Anyone can join! Go ahead and add any papers/comments.

29th July

Christopher VanLang has joined this group

28th July, 2011

Raphael Bruckner

anyway, so that esvelt liu paper, does that actually work now or did they just pretend it works long enough to get that paper published and so that poor kid could graduate?

20th May, 2011

Simon Trevino likes this.

Raphael Bruckner

wait, did i just join a facebook-like online community? oh shit, they got me. they finally got me.

20th May, 2011

Raphael Bruckner has joined this group

20th May, 2011