Peter Nollert, PhD
Director of Membrane Protein Technologies , Emerald BioStructures, Inc.Bainbridge Island, Washington, United States
Research field: Biological Sciences - Biotechnology
Membrane protein crystallization
GPCR structural biology
Publications
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Journal Article (29)
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Ellen Wallace, David Dranow, Philip D. Laible et al. (2011) Monoolein Lipid Phases as Incorporation and Enrichment Materials for Membrane Protein Crystallization, e24488. In PLoS ONE 6 (8).
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Eric R. Smith, Darren W. Begley, Vanessa Anderson et al. (2011) The Protein Maker: an automated system for high-throughput parallel purification, 1015-1021. In Acta Crystallographica Section F Structural Biology and Crystallization Communications 67 (9).
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Liang Li, Qiang Fu, Christopher a Kors et al. (2010) A Plug-Based Microfluidic System for Dispensing Lipidic Cubic Phase (LCP) Material Validated by Crystallizing Membrane Proteins in Lipidic Mesophases., 789-798. In Microfluidics and nanofluidics 8 (6).Download PDF (465.11 KB)
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Cory J. Gerdts, Glenn L. Stahl, Alberto Napuli et al. (2010) Nanovolume optimization of protein crystal growth using the microcapillary protein crystallization system, 1078-1083. In Journal of Applied Crystallography 43 (5).Download PDF (572.79 KB)
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Christopher a Kors, Ellen Wallace, Douglas R Davies et al. (2009) Effects of impurities on membrane-protein crystallization in different systems., 1062-73. In Acta crystallographica. Section D, Biological crystallography 65 (Pt 10).Download PDF (1.37 MB)
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H. Nollert, P, Feese, M, Staker, B., Kim (2007) Protein X-ray crystallography in drug discovery, 1467-70. 37 (8).
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Peter Nollert (2005) Membrane protein crystallization in amphiphile phases: practical and theoretical considerations., 339-57. In Progress in biophysics and molecular biology 88 (3).Download PDF (705.33 KB)
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P Nollert (2004) Climbing Mount Everest, the GPCR way, 2-4. In Drug Discovery Today: TARGETS 3 (1).Download PDF (95.44 KB)
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Peter Nollert (2004) Lipidic cubic phases as matrices for membrane protein crystallization., 348-53. In Methods (San Diego, Calif.) 34 (3).Download PDF (797.69 KB)
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Peter Nollert (2003) Microscope detection options for colorless protein crystals grown in lipidic cubic phases laboratory notes Microscope detection options for colorless protein crystals grown in lipidic cubic phases, 1295-1296. In Nature.Download PDF (340.51 KB)
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Book (1)
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Cory Gerdts, Peter Nollert (2009) CHAPTER 8 Advances in Microfluidic Membrane Protein Crystallization Techniques, 179-189. In Elsevier Inc..Download PDF (332.5 KB)
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Generic (1)
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Nollert_Meth_Enz_figs_2002.pdf.Download PDF (46.53 KB)
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Awards and Grants
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Jul 2005In-Membrane Protein Crystallization
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Apr 1999Long Term Fellow of the Human Frontier Science Program Organization View website
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Dec 1996Distinguished Schloessmann Fellow of the Max-Planck-Society
Biographical Information
Director of Membrane Protein Technologies @ Emerald BioStructures
January 2010 – Present (9 months)
• Membrane Protein Structure Determination (GPCRs, ABC-transporters)
• Membrane protein crystallization research and technology development
• IP, projects, marketing and business development
Director of Emerald BioSystems @ Emerald BioSystems
March 2006 – January 2010 (3 years 11 months)
• Managing operations, research & development and business development activities in the structural proteomics marketplace
• Managing and executing Sales and Marketing, supervising and training new employees
• Managing intellectual property portfolio
• Overseeing product development (crystal imaging, crystallization tools development)
• Managing and hands-on membrane protein crystallization research (GPCRs)
Sr. Research Scientist @ deCODE biostructures
January 2002 – February 2006 (4 years 2 months)
• Hands-on membrane protein structural biology research and development (expression, purification and crystallization; GPCRs)
• Technology development in the area of crystallization robotics, microfluidics and optical polarization microscopy
• Carrying out grant funded basic research and product development driven research (SBRI grant)
• Managing contract research structural biology projects
• Laboratory work: protein engineering, purification, crystallization, binding assay
• Business development worldwide
Postdoc @ UCSF
January 1999 – December 2001 (3 years)
• Crystallization, structure determination and function of the E.coli glycerol channel GlpF
• Development of a high-throughput micro-technology for protein crystallization
• X-ray diffraction experiments at SSRL, ALS, NLS, ESRF
• Quantitative crystallization theory
Postdoc @ Stanford University
January 1999 – April 2001 (2 years 4 months)
• Crystallization of the beta2 adrenergic receptor in lipidic cubic phases
Postdoc @ Biocenter Basel
1997 – 1998 (1 year)
• Membrane protein crystallization in Lipidic Cubic Phases
January 2010 – Present (9 months)
• Membrane Protein Structure Determination (GPCRs, ABC-transporters)
• Membrane protein crystallization research and technology development
• IP, projects, marketing and business development
Director of Emerald BioSystems @ Emerald BioSystems
March 2006 – January 2010 (3 years 11 months)
• Managing operations, research & development and business development activities in the structural proteomics marketplace
• Managing and executing Sales and Marketing, supervising and training new employees
• Managing intellectual property portfolio
• Overseeing product development (crystal imaging, crystallization tools development)
• Managing and hands-on membrane protein crystallization research (GPCRs)
Sr. Research Scientist @ deCODE biostructures
January 2002 – February 2006 (4 years 2 months)
• Hands-on membrane protein structural biology research and development (expression, purification and crystallization; GPCRs)
• Technology development in the area of crystallization robotics, microfluidics and optical polarization microscopy
• Carrying out grant funded basic research and product development driven research (SBRI grant)
• Managing contract research structural biology projects
• Laboratory work: protein engineering, purification, crystallization, binding assay
• Business development worldwide
Postdoc @ UCSF
January 1999 – December 2001 (3 years)
• Crystallization, structure determination and function of the E.coli glycerol channel GlpF
• Development of a high-throughput micro-technology for protein crystallization
• X-ray diffraction experiments at SSRL, ALS, NLS, ESRF
• Quantitative crystallization theory
Postdoc @ Stanford University
January 1999 – April 2001 (2 years 4 months)
• Crystallization of the beta2 adrenergic receptor in lipidic cubic phases
Postdoc @ Biocenter Basel
1997 – 1998 (1 year)
• Membrane protein crystallization in Lipidic Cubic Phases
CV
Professional Experience
2010 - Present
Director of Membrane Protein Technologies at Emerald BioStructures, Inc.
Bainbridge Island, Washington, United States
Bainbridge Island, Washington, United States
Education
Aug 1993 - Jul 1997
Max Planck Institute for Biology
in Tübingen, Germany
Dr. rer. nat
Dr. rer. nat
Apr 1987 - May 1993
Eberhard-Karls-Universität Tübingen
in Tübingen, Germany
Dip. Biochem.
Dip. Biochem.
Consulting Services
Membrane protein crystallization technologies
12/2000 – 12/2001 Emerald BioStructures, Bainbridge Is., WA & MediChem Life Sciences, Inc. Chicago, IL
Membrane protein crystallization technologies
12/2000 – 12/2001 Emerald BioStructures, Bainbridge Is., WA & MediChem Life Sciences, Inc. Chicago, IL
Contact Information
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