An integrated microfluidic culture device for quantitative analysis of human embryonic stem cells
by
Ken-Ichiro Kamei,
Shuling Guo,
Zeta Tak For Yu,
Hiroko Takahashi,
Eric Gschweng,
…
Carol Suh,
Xiaopu Wang,
Jinghua Tang,
Jami McLaughlin,
Owen N Witte,
Ki-Bum Lee,
Hsian-Rong Tseng
show all authors
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Jong Min Lee, Ji-eun Kim, Edward Kang, Sang-Hoon Lee, Bong Geun Chung in Electrophoresis (2011)We developed an integrated microfluidic culture device to regulate embryonic stem (ES) cell fate. The integrated microfluidic culture device consists of an air control channel and a fluidic channel with 44 micropillar arrays. We hypothesized that…Save reference to library · Related research 10 readers
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Laurence M Brill, Wen Xiong, Ki-Bum Lee, Scott B Ficarro, Andrew Crain, Yue Xu, et al. in Cell stem cell (2009)Protein phosphorylation, while critical to cellular behavior, has been undercharacterized in pluripotent cells. Therefore, we performed phosphoproteomic analyses of human embryonic stem cells (hESCs) and their differentiated derivatives. A total of…Save reference to library · Related research 62 readers
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Luis Gerardo Villa-Diaz, Yu-suke Torisawa, Tomoyuki Uchida, Jun Ding, Naiara Correa Nogueira-de-Souza, Kathy Sue O'Shea, et al. in Lab on a Chip (2009)We have developed a miniaturized microfluidic culture system that allows experimentation on individual human embryonic stem cell (hESC) colonies in dynamic (flow applied) or static (without flow) conditions. The system consists of three inlet…Save reference to library · Related research 31 readers
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Heli Skottman, Outi Hovatta in Reproduction Cambridge England (2006)Human embryonic stem cell (hESC) lines have been derived and cultured in variable conditions. The idea behind derivation of hESC lines is to use them in human cell transplantation after differentiation, but already now these cells are widely used…Save PDF to library · Related research 37 readers
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Tenneille E Ludwig, Veit Bergendahl, Mark E Levenstein, Junying Yu, Mitchell D Probasco, James A Thomson in Nature Methods (2006)We recently reported the development of TeSR1, a serum-free, animal productfree medium that supports the derivation and long-term feeder-independent culture of human embryonic stem cells1. Although the derivation of new human embryonic stem cell…Save reference to library · Related research 98 readers
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Andrew L Laslett, Adam A Filipczyk, Martin F Pera in Nature Biotechnology (2003)Human embryonic stem cells have been defined as self-renewing cells that can give rise to many types of cells of the body. How and whether these cells can be manipulated to replace cells in diseased tissues, used to screen drugs and toxins, or…Save PDF to library · Related research 116 readers
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Shuai Wang, Ruijun Tian, Li Li, Daniel Figeys, Lisheng Wang in Proteomics (2011)Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-based quantitative proteomics analysis provides an unprecedented tool for the study of mechanisms underlying the self-renewal and differentiation of human embryonic stem cells (hESCs).…Save reference to library · Related research 11 readers
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Hao Wu, Kevin J Kim, Kshama Mehta, Salvatore Paxia, Andrew Sundstrom, Thomas Anantharaman, et al. in Stem Cells (2008)Differences between individual DNA sequences provide the basis for human genetic variability. Forms of genetic variation include single-nucleotide polymorphisms, insertions/duplications, deletions, and inversions/translocations. The genome of human…Save reference to library · Related research 22 readers
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Wen-hong Zhang, Xiao-fang Sun, Shu Kong, Yong-hua Jiang, Yu-hong Zheng, Yi-fei Yin in Zhonghua yi xue yi chuan xue za zhi Zhonghua yixue yichuanxue zazhi Chinese journal of medical genetics (2007)OBJECTIVE: To establish a protocol for culturing of human embryonic stem cells (HUES4) without any animal-derived feeder cells and to investigate the karyotype stabilities of HUES4 cells after long-term cultivation. METHODS: HUES4 cells were…
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Thomas C Schulz, Anna Maria Swistowska, Ying Liu, Andrzej Swistowski, Gail Palmarini, Sandii N Brimble, et al. in BMC Genomics (2007)Background: Much of our current knowledge of the molecular expression profile of human embryonic stem cells (hESCs) is based on transcriptional approaches. These analyses are only partly predictive of protein expression however, and do not shed…Save PDF to library · Related research 17 readers
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