Papers

DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection. Over the past three years, massively parallel DNA sequencing platforms have become widely available,…

The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to…

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a…

The hallmarks of cancer comprise six biological capabilities acquired during the multistep development of human tumors. The hallmarks constitute an organizing principle for rationalizing the complexities of neoplastic disease. They include…

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform,…

There is increasing concern that most current published research findings are false. The probability that a research claim is true may depend on study power and bias, the number of other studies on the same question, and, importantly, the ratio of…

Ten years after the publication of the position paper The hallmarks of cancer (Hanahan and Weinberg Cell 100:5770, 2000), it has become increasingly clear that mutated cells on their way to giving rise to a tumor have also to learn how to thrive in…

In this article, we attempt to distinguish between the properties of moderator and mediator variables at a number of levels. First, we seek to make theorists and researchers aware of the importance of not using the terms moderator and mediator…

There is increasing concern that most current published research findings are false. The probability that a research claim is true may depend on study power and bias, the number of other studies on the same question, and, importantly, the ratio of…

There is now ample evidence of the ecological impacts of recent climate change, from polar terrestrial to tropical marine environments. The responses of both flora and fauna span an array of ecosystems and organizational hierarchies, from the…

We describe a model-based clustering method for using multilocus genotype data to infer population structure and assign individuals to populations. We assume a model in which there are K populations (where K may be unknown), each of which is…

Computing as you know it is about to change, your applications and documents are going to move from the desktop into the cloud. I'm talking about cloud computing, where applications and files are hosted on a "cloud" consisting of thousands of…

The tutorial starts with an overview of the concepts of VC dimension and structural risk minimization. We then describe linear Support Vector Machines (SVMs) for separable and non-separable data, working through a non-trivial example in detail. We…

Insights into conducting research and the writing of scientific papers are given by Prof. Whitesides in this short essay. The manuscript and its guidelines has been circulated within the Whitesides' research group since 1989.

The dynamic capabilities framework analyzes the sources and methods of wealth creation and capture by private enterprise firms operating in environments of rapid technological change. The competitive advantage of firms is seen as resting on…

We have designed and implemented the Google File System, a scalable distributed file system for large distributed data-intensive applications. It provides fault tolerance while running on inexpensive commodity hardware, and it delivers high…

Error bars commonly appear in figures in publications, but experimental biologists are often unsure how they should be used and interpreted. In this article we illustrate some basic features of error bars and explain how they can help communicate…

Provided certain obstacles are overcome, we believe Cloud Computing has the potential to transform a large part of the IT industry, making software even more attractive as a service and shaping the way IT hardware is designed and purchased.…

Note: OCR errors may be found in this Reference List extracted from the full text article. ACM has opted to expose the complete List rather than only correct and linked references.

We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides…

The complete and specific proteolytic cleavage of protein samples into peptides is crucial for the success of every shotgun LC-MS/MS experiment. In particular, popular peptide-based label-free and targeted mass spectrometry approaches rely on…

Chronic obstructive pulmonary disease (COPD)is a chronic inflammatory lung disease associated vvith Progressive obstruction of aiげ10vv a什ecting perゆheral ai剛ays. The proteomic analysis highlights vvays to identify novel biomarkers for diagnosis,…

Current proteomics technologies based on miniaturized on-line liquid chromatography (LC) coupled with mass spectrometry (MS) allow us to analyze protein expression profiling with a high throughput. Compared to genome sequencing and microarray-based…

Two established but disparate lines of research exist: studies examining the self-stigma associated with mental illness and studies examining the self-stigma associated with seeking psychological help. Whereas some researchers have implicitly…

Respiratory syncytial virus (RSV) is a leading cause of lower respira- tory infection in infants, with nearly all infants infected by the end of their second year. Infections with RSV do not confer completely pro- tective immunity, and reinfections…

Methods for the aromatization of ethyl 6-alkyl-2,4-dioxocyclohexanecarboxylates, available from the base-catalysed condensation between &945;, &946;-unsaturated esters and acetoacetic ester, and related substances have been explored. 5-Nonyl- and…

From the wood of Gevillea striata R. Br., a phenol, striatol, was isolated. Chemical and spectroscopic evidence indicated its structure to be 1,14-bis(3,5-dihydroxy-4-methylphenyl)tetradecane. The structure was confirmed by synthesis.

From the wood of Grevillea robusta a new phenol, grevillol, was isolated in high yield, together with sitosterol. The structure of grevillol was established as 5-n-tridecylresorcinol by spectroscopic methods, chemical degradation, and synthesis. The…

Macadamia jansenii is endemic to south-east Queensland, Australia, and is currently known from a single population 180km north of the nearest wild population of its congener, the edible Macadamia integrifolia. A recently developed Macadamia recovery…

Bereaved people are at a greater risk of losing their lives than the non-bereaved. This paper explores the evidence on this "bereavement-mortality relationship," to establish who among the bereaved are most vulnerate to dire consequences of loss...

Reports on a survey of undergraduates at a large southeastern university about their recovery from a love relationship that ended. Sex differences on the degree of difficulty of their recovery; Effect of time and a new partner on getting over a…

The escape rate of surface state electrons on liquid helium was measured in the temperature interval 0.56<T<1.0 K for electron areal density below 5107 cm2, where the rate is independent of density. It is found that the escape rate is dominated by…

This article features Advanced Competitive Science (ACS), a two-year course introduced by a science teacher, Joe Pouliot, in 2004 at Trinity High School in Manchester, New Hampshire. More than a traditional STEM (science, technology, engineering,…

Bereaved people are at a greater risk of losing their lives than the non-bereaved. This paper explores the evidence on this "bereavement-mortality relationship," to establish who among the bereaved are most vulnerate to dire consequences of loss...

Bereaved people are at a greater risk of losing their lives than the non-bereaved. This paper explores the evidence on this "bereavement-mortality relationship," to establish who among the bereaved are most vulnerate to dire consequences of loss...

Bereaved people are at a greater risk of losing their lives than the non-bereaved. This paper explores the evidence on this "bereavement-mortality relationship," to establish who among the bereaved are most vulnerate to dire consequences of loss...

Two established but disparate lines of research exist: studies examining the self-stigma associated with mental illness and studies examining the self-stigma associated with seeking psychological help. Whereas some researchers have implicitly…

Reports on a survey of undergraduates at a large southeastern university about their recovery from a love relationship that ended. Sex differences on the degree of difficulty of their recovery; Effect of time and a new partner on getting over a…

Two established but disparate lines of research exist: studies examining the self-stigma associated with mental illness and studies examining the self-stigma associated with seeking psychological help. Whereas some researchers have implicitly…

Bereaved people are at a greater risk of losing their lives than the non-bereaved. This paper explores the evidence on this "bereavement-mortality relationship," to establish who among the bereaved are most vulnerate to dire consequences of loss...