An immunocytochemical method using specific antibodies was employed to detect DNA polymerase alpha-primase complex in Drosophila melanogaster embryos during the first 13 nuclear division cycles. A monoclonal antibody specific to the 72 kDa…
Papers in Chromatography
Chromatography papers in Chemistry, A
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Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation of A14-125Imonoiodoinsulin directly from the iodination mixtures. It remains to be clarified, however, whether the RP-HPLC chromatographic conditions affect…
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This work examines the main features of modern electronic noses (e-noses) and their most important applications in food control in this new century. The three components of an electronic nose (sample handling system, detection system, and data…
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Neuraminidase activity of influenza virus was directly seen on sodium dodecyl sulfate polyacrylamide gels with the aid of the synthetic substrate, methoxyphenol neuraminic acid. Neuraminidase (NA) appeared as a high-molecular-weight fraction with a…
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A32390A is an isonitrile-containing derivative of diacyl D-mannitol. The compound is produced in fermentation as the major component of a metabolic complex known as A32390. A32390A inhibits dopamine-beta-hydroxylase reduces heart and adrenal…
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A four-step procedure for purification of a nuclease from the keratinous sponge Verongia aerophoba is described. The extracted material is lyophilized, acidified, and subjected to chromatography on Sephadex, hydroxyapatite, and phosphocellulose. The…
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A tridecapeptide containing tritium-labelled lysine and corresponding closely to residues 98 to 110 of the alpha chain of type I collagen was synthesized by the solid-phase method. Gly-Leu-Hyp-Gly-Nle-4,5-3HLys-Gly-His-Arg-Gly-Phe-Ser-Gly was used…
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A strain of Pseudomonas putida grown on 4-methoxybenzoate as sole carbon source contains an enzyme system for the O-demethylation of this substrate. The enzyme system is purifiable and can be separated into two components: an NADH-dependent…
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A and B and A1Leb substances were adsorbed onto red cells exposed to glycosphingolipid fractions prepared from the serum of group A and B and A1,Le(a minus b plus) donors. Group O cells exposed to fractions prepared from the serum of group A or B…
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Variable selection has been discussed in many papers and it became an important topic in areas as chemometrics and science in general. Here a backward iterative step-by-step wrapper method is proposed using PLS. The root-mean-square error of…
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Adipic acid (AA) is the main intermediate in nylon 6, 6 that is manufactured by polymerization condensation of AH salt (hexamethylenediammonium adipate). Adipic acid is also an intermediate in the production of polyester-polyol, a material used in…
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We conducted this study to determine efficacy of Parasight-F (an HRP-II antigen dipstick method to detect P. Falciparum) in children. A total of 30 children were enrolled in the age group of 2 months to 12 years whose peripheral smear showed asexual…
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Blue belt: Condensation polymerization of pyrene (blue) and triphenylene (green) monomers leads to the formation of a hexagonal mesoporous covalent organic framework (see picture). This material exists in a belt shape, absorbs photons over a wide…
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A Bence Jones protein with cryoglobulin properties has been isolated from the urine of a myeloma patient. The Bence Jones cryoglobulin was of type K and had a sedimentation coefficient of 35S at a concentration of 5 mg/ml. The concentration…
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A bench-scale study on swine manure stabilization for odour control was conducted using batch aeration reactors. In trial 1, two aeration lengths, i.e., 0.5 and 4.0 day, were used under uncontrolled ambient temperature that increased gradually over…
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Coniferin, the glucoside of the monolignol coniferyl alcohol, accumulates to high levels in gymnosperms during spring-cambial reactivation. A cinnamyl alcohol glucoside/beta-glucosidase system is thought to play a key role in lignification by…
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Two distinct DNA polymerases (DNA nucleotidyltransferases) have been partially purified from exponentially growing cells of the slime mold Dictyostelium discoideum. The major DNA polymerase activity, DNA polymerase A, has physical and enzymatic…
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Many plants synthesize and accumulate proline in response to osmotic stress. Despite the importance of this pathway, however, the exact metabolic route and enzymes involved in the synthesis of proline in plants have not been unequivocally…
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Two isozymes of chorismate mutase (CA mutase(1) and CA mutase(2)) and two isozymes of prephenate dehydratase (PPA dehydratase(1) and PPA dehydratase(2)) have been found in Pseudomonas aeruginosa. The activities CA mutase(2)-PPA dehydratase(2)…
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Ab initio calcns. show that Ca can decorate org. linkers of metal-org. framework, MOF-5, with a binding energy of 1.25 eV. The Ca-decorated MOF-5 can store mol. hydrogen (H2) in both high gravimetric (4.6 wt.%) and high volumetric (36 g/L)…
Papers in associated sub-disciplines
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