Association of CASP3 and CASP9 polymorphisms with genetic susceptibility to stomach cancer

Abstract

Objective: To investigate the association between the apoptosis genes CASP3 (rsl2108497) and CASP9 (rs4646018) polymorphisms and the risk of developing stomach cancer. Methods: In this population-based case-control study, 278 cases with stomach cancer and 278 age (±5 years), gender, and residential area matched controls were recruited. The genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The unconditional Logistic regression analysis was utilized to calculate the odds ratios (OR) and 95% confidence intervals (CI). Results: The individuals with TC, CC genotypes of rs12108497 locus had significantly increased risk of stomach cancer in comparison to those carrying TT genotype (OR= 1.45, 95% CI: 1.01-2.07 for TC; OR = 2. 17, 95%CI: 1. 15-4. 08 for CC). However, the rs4646018 locus of CASP9 gene polymorphism was not related to stomach cancer risk. Compared with the subjects carrying the both low-risk genotypes, those carrying 1 or 2 high-risk genotypes had a noteworthy increased risk of stomach cancer (OR= 1. 60, 95% CI= 1. 12-2. 30). The combined high-risk genotypes appeared to be more evident in subjects of male (OR=1. 62, 95% CI= 1.05-2.49), ever-smokers (OR=1.87, 95%CI= 1. 12-3. 12), ever-drinkers (OR=1.92, 95%CI = 1.02-3. 65) and no family history of cancer (OR=1. 78, 95%CI= 1. 18-2. 68). Conclusion: The current findings suggest that the polymorphism of CASP3 rs12108497 might be associated with the risk of stomach cancer. However, the CASP9 rs4646018 polymorphism may not be related to the stomach cancer risk.