Retinochoroiditis manifests in patients infected with Toxoplasma gondii. Here, we assessed 30 sibships and 89 parent/case trios of presumed ocular toxoplasmosis (POT) to evaluate associations with polymorphisms in the NOD2 gene. Three haplotype-tagging single-nucleotide polymorphisms (tag-SNPs) within the NOD2 gene were genotyped. The family-based association test showed that the tag-SNP rs3135499 is associated with retinochoroiditis (P = .039). We then characterized the cellular immune response of 59 cases of POT and 4 cases of active ocular toxoplasmosis (AOT). We found no differences in levels of interferon γ (IFN-γ) and interleukin 2 produced by T-helper 1 cells when comparing patients with AOT or POT to asymptomatic individuals. Unexpectedly, we found an increased interleukin 17A (IL-17A) production in patients with POT or OAT. In patients with POT or AOT, the main cellular source of IL-17A was CD4+CD45RO+T-bet-IFN- γ- T-helper 17 cells. Altogether, our results suggest that NOD2 influences the production of IL-17A by CD4+ T lymphocytes and might contribute to the development of ocular toxoplasmosis. © The Author 2012.
CITATION STYLE
Dutra, M. S., Béla, S. R., Peixoto-Rangel, A. L., Fakiola, M., Cruz, A. G., Gazzinelli, A., … Gazzinelli, R. T. (2013). Association of a NOD2 gene polymorphism and T-helper 17 cells with presumed ocular toxoplasmosis. Journal of Infectious Diseases, 207(1), 152–163. https://doi.org/10.1093/infdis/jis640
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