Association of SRD5A2 variants and serum androstane-3alpha,17beta-diol glucuronide concentration in Chinese elderly men

Abstract

BACKGROUND: Results of recent studies have demonstrated that genetic variants of the enzyme steroid 5alpha reductase type II (SRD5A2) are associated with serum concentrations of major androgen metabolites such as conjugates of androstane-3alpha,17beta-diol-glucuronide (3alpha-diol-G). However, this association was not consistently found among different ethnic groups. Thus, we aimed to determine whether the association with SRD5A2 genetic variations exists in a cohort of healthy Chinese elderly men, by examining 2 metabolite conjugates: androstane-3alpha,l7beta-diol-3-glucuronide (3alpha-diol-3G) and androstane-3alpha,17beta-diol-17-glucuronide (3alpha-diol-17G). METHODS: We used GC-MS and LC-MS tomeasure serum sex steroid concentrations, including testosterone and dihydrotestosterone, and 3alpha-diol-3G and 3alpha-diol-17G in 1182 Chinese elderly men age 65 and older. Genotyping of the 3 SRD5A2 tagSNPs [rs3731586, rs12470143, and rs523349 (V89L)] was performed by using melting-temperature-shift allele-specific PCR. RESULTS: The well-described SRD5A2 missense variant rs523349 (V89L) was modestly associated with the 3alpha-diol-17G concentration (P = 0.040). On the other hand, SNP rs12470143 was found to be significantly correlated with 3alpha-diol-3G concentration (P = 0.021). Results of haplotype analysis suggested that the presence of an A-C-G haplotype leads to an increased 3alpha-diol-3G concentration, a finding consistent with results of single SNP analysis. CONCLUSIONS: The genetic variation of SRD5A2 is associated with circulating 3alpha-diol-3G and 3alpha-diol-17G concentrations in Chinese elderly men. In addition, we showed that SRD5A2 haplotypic association, rather than a single SNP alone, might be a better predictor of the 3alpha-diol-G concentration. Thus, the effect of either the haplotype itself or of other ungenotyped SNPs in linkage disequilibrium with the haplotype is responsible for the interindividual variation of 3alpha-diol-G. © 2010 American Association for Clinical Chemistry.