A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A260-A320):(A280-A320) ratio of the extracted DNA was 1.9 ± 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods. © 2006 Elsevier B.V. All rights reserved.
CITATION STYLE
Ota, H., Lim, T. K., Tanaka, T., Yoshino, T., Harada, M., & Matsunaga, T. (2006). Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles. Journal of Biotechnology, 125(3), 361–368. https://doi.org/10.1016/j.jbiotec.2006.03.007
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