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BA enhances the germination of oil palm somatic embryos derived from embryogenic suspension cultures

by Laboratoire Gene, Trop Ird, Montpellier Cedex
Plant Cell (1999)

Abstract

Embryogenic suspension cultures of oil palm(Elaeis guineensis Jacq.) allowmass propagation of somatic embryos; however regeneration rates are low. Histological observations have revealed that shoot development might be limited by the absence of a caulinary meristem. The addition of 6-benzyladenine during development was found to induce shoot apex differentiation and thus increased germination rates, by up to 70%. However, multiple shoot formation was a consequence of a longer period of cytokinin supply during the development of the embryo. In contrast, a short period of culture on medium with 6-benzyladenine at the begining of embryo development was found to result in single shoot production.

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