Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: A preliminary study

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Abstract

Purpose: To identify reliable genomic biomarkers expressed in cumulus cells that accurately and non-invasively predict the oocyte developmental competence and reinforce the already used morphological criteria. Methods: Eight consenting patients were selected for ovarian stimulation and ICSI procedures. Cumulus-oocyte complexes were transvaginally punctured and individually selected based on both good morphological criteria and high zona pellucida birefringence. Following ICSI, two 3-day embryos per patient were transferred. Pregnancy outcome was recorded and proven implantation was thereafter confirmed. Differential gene expression was assessed using two microarray platforms. Further real-time PCR validation, Ingenuity pathways analysis and intra-patient analysis were performed on 17 selected candidates. Results: Seven genes were differentially (p≤0.05) associated to successful pregnancy and implantation. These biomarkers could be used to predict the oocyte developmental competence. Conclusions: These genomic markers are a powerful reinforcement of morphological approaches of oocyte selection. Their large-scale validation could increase pregnancy outcome and single embryo transfer efficiency. © 2010 Springer Science+Business Media, LLC.

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Assidi, M., Montag, M., Van Der Ven, K., & Sirard, M. A. (2011). Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: A preliminary study. Journal of Assisted Reproduction and Genetics, 28(2), 173–188. https://doi.org/10.1007/s10815-010-9491-7

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