A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-β-ionone, a C 13-norisoprenoidic compound, and a C14-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C13-norisoprenoid level increased from véraison to maturity in both cultivars.
CITATION STYLE
Mathieu, S., Terrier, N., Procureur, J., Bigey, F., & Günata, Z. (2005). A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: Functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation. Journal of Experimental Botany, 56(420), 2721–2731. https://doi.org/10.1093/jxb/eri265
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