Changes in cytosolic free calcium concentration in isolated rat parotid cells by cholinergic and beta-adrenergic agonists.
- PubMed: 2413853
Abstract
The alteration in the concentration of cytosolic free calcium (Ca2+i) in isolated rat parotid cells caused by autonomic agents was directly measured using the Ca-sensitive fluorescent probe, quin2. Ca2+i of unstimulated cells was estimated to be 162.7 3.2 nM in normal medium. Carbachol (CCh) and isoproterenol (ISP) caused a rapid rise in Ca2+i in a dose-dependent manner. Maximum increases in Ca2+i induced by CCh and ISP were approximately 100% and 25% of resting level, respectively. In Ca-free medium, CCh produced a small, rapid rise in Ca2+i, followed by a slow decay and a return to resting level within 3-4 min, while all doses of ISP tested failed to change Ca2+i. These results suggest that CCh mobilizes Ca2+ from both extracellular and intracellular pools and then results in a rise in Ca2+i, whereas ISP may slightly mobilize only the extracellular Ca pool.
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