A synthetic deoxyribonucleotide probe for virulent Listeria monocytogenes, designated ADO7, was evaluated for its ability to identify restriction fragments of L. monocytogenes with nucleic acid sequences homologous with the β-hemolysin gene by Southern blot hybridization of clinical and food isolates. The synthetic probe hybridized with three restriction fragments (approximately 1.1, 0.86, and 0.76 kb) of the serotype 1/2A isolates. Southern blot hybridization of the serogroup 4B isolates indicated that the nucleic acid sequences homologous with the β-hemolysin gene probe were limited to a single restriction fragment of approximately 1 kb. © 1990.
CITATION STYLE
Wesley, I. V., Wesley, R. D., Heisick, J., Harrell, F., & Wagner, D. (1990). Characterization of Listeria monocytogenes isolates by Southern blot hybridization. Veterinary Microbiology, 24(3–4), 341–353. https://doi.org/10.1016/0378-1135(90)90182-U
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