Chondrogenic Differentiation Potential of Human Dedifferentiated Fat (DFAT) Cells

Abstract

246 要旨 dedifferentiated fat cell (DFAT) DFAT DFAT 3 II 3 DFAT 2 DFAT は じ め に 原 著 1) 2) 3) 1) 1) 2) 3) Dedifferentiated fat (DFAT) cells are multipotent cells that can be prepared from mature adipocytes by the ceiling culture method. In the present study, we examined the chondrogenic differentiation potential of human DFAT cells. We employed a pellet culture system to induce chondrogenic differentiation. After 3 weeks of pellet culture, DFAT cells formed cartilaginous micromass pellets that were positive for aggrecan and type II collagen. To test whether DFAT cell-derived micromass pellets could form cartilage-like tissue in vivo, the pellets were implanted subcutaneously into immuno-deficient SCID mice. Two weeks after transplantation, the removed implants exhibited well-differentiated elastic cartilage-like tissue. These results indicate that human DFAT cells exhibit high chondrogenic differentiation potential both in vitro and in vivo. DFAT cells may be an attractive cell source for cell-based therapy for cartilage tissue regeneration.