G-protein coupled receptors (GPCRs) play a key role in physiological processes and are attractive drug targets. Their biophysical characterization is, however, highly challenging because of their innate instability outside a stabilizing membrane and the difficulty of finding a suitable expression system. We here show the cell-free expression of a GPCR, CXCR4, and its direct embedding in diblock copolymer membranes. The polymer-stabilized CXCR4 is readily immobilized onto biosensor chips for label-free binding analysis. Kinetic characterization using a conformationally sensitive antibody shows the receptor to exist in the correctly folded conformation, showing binding behaviour that is commensurate with heterologously expressed CXCR4. Copyright:
CITATION STYLE
De Hoog, H. P. M., JieRong, E. M. L., Banerjee, S., Décaillot, F. M., & Nallani, M. (2014). Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. PLoS ONE, 9(10). https://doi.org/10.1371/journal.pone.0110847
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