Chromatin-associated RNA (caRNA) is a newly identified class of RNA molecules stably associated with chromatin, maintaining the higher order structure of euchromatic regions in an accessible form (Schubert et al., Mol Cell, doi:10.1016/j.molcel.2012.08.021, 2012). This section provides a detailed protocol describing the isolation of small caRNA from Drosophila cells and preparation of libraries suited for stranded small (20-200 bp) RNA deep sequencing on the Illumina platform.
CITATION STYLE
Diermeier, S. D., Schubert, T., & Längst, G. (2014). Deep Sequencing of Small Chromatin-Associated RNA: Isolation and Library Preparation (pp. 343–353). https://doi.org/10.1007/978-1-62703-706-8_27
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