We describe DNase-capture, an assay that increases the analytical resolution of DNase-seq by focusing its sequencing phase on selected genomic regions. We introduce a new method to compensate for capture bias called BaseNormal that allows for accurate recovery of transcription factor protection profiles from DNase-capture data. We show that these normalized data allow for nuanced detection of transcription factor binding heterogeneity with as few as dozens of sites.
CITATION STYLE
Kang, D., Sherwood, R., Barkal, A., Hashimoto, T., Engstrom, L., & Gifford, D. (2017). DNase-capture reveals differential transcription factor binding modalities. PLoS ONE, 12(12). https://doi.org/10.1371/journal.pone.0187046
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