The objective of this study was to evaluate the effects of coadministration of ractopamine-HCl (RAC) and trenbolone acetate plus estradiol (TBA) on LM fiber cross-sectional area (CSA), diameter, fiber-associated myonuclei, and satellite cell number. Culled crossbred beef cows (n = 98; 11 ± 1.8 yr old; BCS 4.3 ± 0.03) from a single ranch in south Florida were fed a concentrate diet for 92 d in a 2 × 2, randomized block design. Cows were blocked by BW on arrival into light (initial BW = 369.75 ± 2.68 kg and end BW = 501.96 ± 6.90 kg) and heavy (initial BW = 418.31 ± 2.75 kg and end BW = 522.15 ± 7.09 kg) groups before assignment to treatment. Factors included dietary treatment (0 or 15 ppm) and implant status (0 or 80 mg of trenbolone acetate + 16 mg of estradiol). Ractopamine was provided in the diet to 2 pens or half the treatments during the final 35 d of feeding. Cows were slaughtered on d 92. Forty-eight hours postmortem, the 6th-rib portions of the LM were obtained from 10 randomly selected carcasses from each treatment group (n = 40). Cryosections (12 μm) were immunostained for dystrophin and myosin heavy chain I or II for the measurement of fiber CSA and type, respectively. Fiber-associated nuclei and satellite cell numbers were measured in serial cryosections. There was a RAC × TBA interaction (P < 0.05). Type I fiber CSA and diameter were increased (P < 0.05) by TBA and RAC. Type ICSA and diameter were larger (P < 0.05) in TBA + RAC than RAC only. Type II fiber CSA and diameter were not affected by TBA (P = 0.48), RAC (P = 0.15), or TBA + RAC (P = 0.60). Satellite cell numbers and fiber-associated nuclei were not affected (P > 0.05) by implant status or ractopamine supplementation. These results indicate that TBA and RAC preferentially increase the size of type I fibers in cull cows. ©2007 American Society of Animal Science. All rights reserved.
CITATION STYLE
Gonzalez, J. M., Carter, J. N., Johnson, D. D., Ouellette, S. E., & Johnson, S. E. (2007). Effect of ractopamine-hydrochloride and trenbolone acetate on longissimus muscle fiber area, diameter, and satellite cell numbers in cull beef cows. Journal of Animal Science, 85(8), 1893–1901. https://doi.org/10.2527/jas.2006-624
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