Effects of fungicides JS399-19, azoxystrobin, tebuconazloe, and carbendazim on the physiological and biochemical indices and grain yield of winter wheat

Abstract

The impact of four modern fungicides JS399-19 (2-cyano-3-amino-3-phenylancryic acetate) (novel fungicide), azoxystrobin (a strobilurin), tebuconazole (a triazole) and carbendazim (a benzimidazole), applied as foliar spray at the recommended field rate, on the physiology and biochemistry of the senescence process and grain yield was studied in winter wheat (Triticum aestivum L. cv. 'Nannong No. 9918') under natural environmental conditions. Fungicide treatments to wheat plants at growth stage [ZGS] 57 (3/4 of head emerged) significantly increased the chlorophyll (CHL) and soluble protein (SP) content and decreased the malondialdehyde (MDA) content and electrolyte leakage. Additionally, activities of the antioxidative enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in flag leaves of the fungicide-treated plants were also higher than that in untreated plants. These coincided with elevated levels of H2O2 and reduced level of O2- in the fungicide-treated plants. The results suggested that the fungicide-induced delay of senescence was due to an enhanced antioxidant enzyme activity protecting the plants from harmful active oxygen species (AOS). Because all fungicides can induce the delay of wheat senescence, fungicide-treated wheat shown higher grain yield than untreated wheat. Of all tested fungicides, JS399-19, azoxystrobin and tebuconazole showed similar effects on delaying senescence of wheat and enhancing the grain yield of wheat, but JS399-19 was more efficient in general. © 2010 Elsevier Inc.