Four-stranded G-quadruplex DNA secondary structures have recently been visualized in the nuclei of human cultured cells. Here, we show that BG4, a G-quadruplex-specific antibody, can be used to stain DNA G-quadruplex structures in patient-derived tissues using immunohistochemistry. We observe a significantly elevated number of G-quadruplex-positive nuclei in human cancers of the liver and stomach as compared to background non-neoplastic tissue. Our results suggest that G-quadruplex formation can be detected and measured in patient-derived material and that elevated G-quadruplex formation may be a characteristic of some cancers. © 2014 Biffi et al.
CITATION STYLE
Biffi, G., Tannahill, D., Miller, J., Howat, W. J., & Balasubramanian, S. (2014). Elevated levels of G-quadruplex formation in human stomach and liver cancer tissues. PLoS ONE, 9(7). https://doi.org/10.1371/journal.pone.0102711
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