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Enumeration of bacteriophages by double agar overlay plaque assay.

by Andrew M Kropinski, Amanda Mazzocco, Thomas E Waddell, Erika Lingohr, Roger P Johnson
Methods in molecular biology (Clifton, N.J.) ()

Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

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