Feasibility of using a surface plasmon resonance-based biosensor to detect and quantify yessotoxin

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Abstract

Yessotoxin (YTX) is a disulfated polyether toxin produced by marine dinoflagellates. Although there is no clear evidence that YTX is toxic to humans, it is a major cause of false positives in DSP toxin detection by mouse bioassay. We developed a new detection and quantification method for yessotoxin using a BiaCore X Surface plasmon resonance (SPR)-based biosensor. The assay is based in the interaction of YTX with phosphodiesterase enzymes (PDE), one of its cellular targets. The injection of several YTX concentrations (3-12 μM) over immobilized PDE I, showed a dose dependent binding signal, which Kobs (observed rate constant) allowed us to obtain a calibration curve with a linear fit. The detection of yessotoxin using SPR-based biosensor allows the quantification of the toxin with an automated and repetitive method at concentrations in the range of the 1 mg kg-1 European regulatory limit. © 2008 Elsevier B.V. All rights reserved.

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Fonfría, E. S., Vilariño, N., Vieytes, M. R., Yasumoto, T., & Botana, L. M. (2008). Feasibility of using a surface plasmon resonance-based biosensor to detect and quantify yessotoxin. Analytica Chimica Acta, 617(1–2), 167–170. https://doi.org/10.1016/j.aca.2008.01.010

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