A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimate plasmid copy number by densitometry is also described. © 1994 Academic Press, Inc.
CITATION STYLE
Coronado, C., Vázquez, M. E., Cebolla, A., & Palomares, A. J. (1994). Use of firefly luciferase gene for plasmid copy number determination. Plasmid, 32(3), 336–341. https://doi.org/10.1006/plas.1994.1074
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