New fluorogenic sensors with suitable kinetic parameters and sensitivity have been developed for the determination of sphingosine-1-phosphate lyase activity in cell lysates. The probe RBM148 can be efficiently loaded into cationic liposomes and used to determine S1PL activity in intact cells.
CITATION STYLE
Sanllehí, P., Casasampere, M., Abad, J. L., Fabriàs, G., López, O., Bujons, J., … Delgado, A. (2017). The first fluorogenic sensor for sphingosine-1-phosphate lyase activity in intact cells. Chemical Communications, 53(39), 5441–5444. https://doi.org/10.1039/c7cc01659j
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