Fluorescence In Situ Hybridization

Abstract

Fluorescence in situ hybridization (FISH) is a method of using fluorescent probes to detect specific nucleic acid sequences within cells and their location. It can be used to detect a variety of cytogenetic variations, including chromosomal deletions, amplification, and translocation. As a classical cytogenetic method, FISH is widely used in prenatal diagnosis as well as the detection of tumors and infections. FISH is a combination of cytogenetics and molecular biology. It is a bridge between cytogenetics and molecular biology and belongs to molecular cytogenetics. FISH is fast, safe, with high sensitivity, and is easy to use.