An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae
The inoculating technique consists of clipping off the tips of rice leaves with scissors whose blades have been dipped in bacterial suspension. Five 60- to 80-day-old plants can be sucessively inoculated after dipping into a suspension containing 109 cells/ml. Although the technique primarily measures tissue susceptibility, a good correlation exists between this and natural infection. Compared with earlier methods, this one is more efficient, allows diseased leaf extract as an alternative to pure cultures) to be more easily used as inoculum and gives more consistent results.