Objective: To investigate the impact of injection speeds of the transferred load on embryo viability. Design: Laboratory model for in vitro simulation of embryo transfer (ET). Setting: Academic research institutes of reproduction biotechnology and private centers of reproductive medicine. Animal(s): Mouse hybrid F1 females, C57bl/10J × CBA-H (N = 15), aged 2 to 3 months. Intervention(s): In vitro exposure of mouse blastocysts to either fast ET with an ejection speed of the transferred load of >1 m/s or slow ET with an ejection speed of <0.1 m/s. Main Outcome Measure(s): Morphologic changes and apoptotic index of blastocysts. Result(s): Morphologic changes in response to ET were most prevalent in blastocysts exposed to fast ET. The mean apoptotic index was 52% in the group exposed to fast ET, 25% in the group exposed to slow ET, and 12.8% in control group. Conclusion(s): Fast ejection of the transferred load can trigger both morphologic changes and apoptosis in mouse blastocysts. A reduction of the ejection speed of the transferred load minimizes injury to the embryos. Therefore, embryos should be transferred at the lowest possible speed. © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.
CITATION STYLE
Grygoruk, C., Sieczynski, P., Modlinski, J. A., Gajda, B., Greda, P., Grad, I., … Mrugacz, G. (2011). Influence of embryo transfer on blastocyst viability. Fertility and Sterility, 95(4), 1458–1461. https://doi.org/10.1016/j.fertnstert.2010.04.073
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