Instructions for use studies on plant cell and tissue culture

Abstract

Since Guha and Maheshwari (1964, 1966) succeeded in producing haploid plants by anther culture of Datura innoxia in vitro, a number of workers have attempted to apply anther culture to a wide species of plants. This is because haploid plants are generally recognized for their usefulness for various approaches in cytogenetics, genetics, and plant breeding works. Thus to the present, a considerable amount of success of producing haploid plants by anther culture have been reported for other species: e. g. Nicotiana species (BOURG IN and NITSCH, 1967; NAKATA and TANAKA, 1968; SUNDERLAND and WICKS, 1969 a, b), Oryza sativa (NrrzEKI and OONO, 1968; NISHI and MITSUOKA, 1969; HARN, 1969; GUHA et al., 1970), Brassica (KAMEYA and HINATA, 1970), Lolium multiflorum x Festuca arundinacea (NITZSCHE, 1970), Lolium multiflorum and Hordeum vulgare (CLAPHAM, 1971) Datura metel (NARAYANASWAMY and CHANDY, 1971), Atropa belladonna (ZENKTELER, 1971), and Aegilops (KIMATA and SAKAMOTO, 1972). The present paper gives the result of work aimed at inducing haploid plants of forage crops belonging to Gramineae and Leguminosae which is of considerable economic importance.